Paipu CPT 8ml
ʻO kahi Vacutainer CPT (Cell Preparation Tube) 8mL he paipu hōʻiliʻili koko kūikawā i hoʻohana ʻia no ka hoʻokaʻawale ʻana i nā pūnaewele mononuclear (PBMC) mai ke koko holoʻokoʻa. Hoʻohana maʻamau ia i ka immunology, ka noiʻi ʻana i nā maʻi infectious, a me nā noi lapaʻau e like me ka nānā ʻana i ka immune a me nā hoʻāʻo e pili ana i ke kelepona.
ʻO nā hiʻohiʻona nui o ka Vacutainer CPT Tube (8mL):
Hoʻopiha mua ʻia me ka mea anticoagulant a me ka gradient medium
Loaʻa iā Sodium Citrate ma ke ʻano he anticoagulant.
Loaʻa iā Ficoll™-based density gradient separation gel e hoʻomaʻamaʻa i ka hoʻokaʻawale ʻana o nā cell mononuclear.
Hiki ke hoʻokaʻawale i hoʻokahi ʻanuʻu PBMC
Hoʻopau i ka pono no ka manual density gradient centrifugation (eg, Ficoll-Paque™).
Hoʻemi i ka manawa hana a me ka loli.
Ka nui o ke koko ma'amau
Hoʻolālā ʻia e hana i ka 8mL o ke koko holoʻokoʻa.
Paʻa, paipu hoʻohana hoʻokahi
Hoʻohana ʻia no nā noi lapaʻau a me nā noiʻi noiʻi kahi e pono ai ke kaʻawale PBMC kiʻekiʻe.
He kūpono me ka centrifugation
Pono kahi protocol centrifugation kūikawā e hoʻokaʻawale maikaʻi i nā PBMC mai nā mea koko ʻē aʻe.
Hoʻohana a me nā noi:
Hoʻokaʻawale ʻia ʻo PBMC no nā haʻawina hana immune (nā pūnaewele T, nā pūnaewele B, nā pūnaewele NK, nā monocytes).
Flow cytometry, ELISPOT, cytokine assays, a me nā hoʻoponopono immunological ʻē aʻe.
ʻO ka noiʻi ʻana i ka maʻi lele a me ka lāʻau lapaʻau.
Ka nānā ʻana i ka maʻi autoimmune.
ʻO nā hoʻokolohua lapaʻau a me ka ʻike biomarker.
Pehea e hana ai (Ke kaʻina hana kumu):
1. E hōʻiliʻili i ka laʻana koko i loko o ka paipu CPT (hoʻopiha i ka leo i koho ʻia).
2. E hoʻohuli mālie (8-10 manawa) e hui pū me ka anticoagulant.
3. ʻO Centrifuge e like me ka protocol a ka mea hana (maʻamau ma kahi o 1500-1800 RCF no 20-30 mau minuke ma ka wela lumi).
4. Hoʻokumu ka papa PBMC ma luna o ka mea hoʻokaʻawale gel ma hope o ka centrifugation.
5. E wehe pono i ka papa PBMC me ka hoʻohana ʻana i ka pipette no nā noi lalo.
Ka mālama ʻana a me ka mālama ʻana:
E mālama i ka lumi wela (15–25°C).
E pale i ka wela nui a i ʻole ka hau.
